Dr. Antonio Brunetti, M.D., Ph.D
Personal Information
Name :
Antonio Brunetti
Marital
status : Married
Wife :
Daniela P. Foti
Children :
Francesco Saverio, age 12
Mattia
Angelo, age 10
Corresponding
Address :
Dept. of Clinical and Experimental Medicine
University of Catanzaro “Magna Græcia”
Via T. Campanella, 115
88100 Catanzaro
Italy
Tel :
+39 0961-712257
Fax : +39 0961-775373
Education and Training :
1983 : MD degree and laurea in Medicine at the University of Catania, Catania, Italy;
1986 : Specialty in Endocrinology and Metabolism at the University of Catania, Catania, Italy;
1992 : Ph.D. degree in Endocrinology and Metabolism at the University of Naples, Naples, Italy;
1993 to date : Professor in Endocrinology and Metabolism at the University of Catanzaro “Magna Græcia”, Catanzaro, Italy.
Employment and Research Experience:
1993 to date : Professor in Endocrinology and Metabolism at the University of Catanzaro “Magna Græcia”, Catanzaro, Italy;
1996 : Associate Specialist at the Diabetes & Endocrine Research Laboratory, University of California San Francisco (UCSF), San Francisco, CA, USA;
1993 : San Francisco, Associate Specialist at the Diabetes and Endocrine Research Laboratory, University of California San Francisco (UCSF), San Francisco, CA, USA;
1986-1992 : Visiting Specialist at the Diabetes and Endocrine Research Laboratory, University of California San Francisco (UCSF), San Francisco, CA, USA;
1985 : Visiting Fellow at the National Council of Research (CNR), University of Pisa, Pisa, Italy;
1983-1984 : Postdoctoral Fellow at the Department of Endocrinology and Metabolism, University of Catania, Catania, Italy.
Research Area :
•
Phatophisiology of insulin action
• Insulin resistance
• Obesity
• Diabetes
Conference presentations:
1988 New Orleans (USA) : The American Endocrine Society, 70th AnnualMeeting
1989 Detroit (USA) : The American Diabetes Association, 49th Annual Meeting
1990 Atlanta (USA) : The American Endocrine Society, 72th Annual Meeting
1991 San Francisco (USA) : 2nd International Symposium on Insulin-Like Growth Factors Somatomedins
1991 Washington D.C. (USA) : The American Endocrine Society, 73rd Annual Meeting
1992 San Antonio (USA) : The American Diabetes Association, 52nd Annual Meeting
1992 Rapallo (Italy) : 3rd International Symposium on Endocrinology Under 35
1993 Munich (Germany) : V International Symposium on Insulin Receptor and Insulin Action
1994 Volterra (Italy) : International Symposium on “Where phenotype does not match genotype”
1997 Bologna (Italy) : VI Telethon Convention
1998 Roma (Italy) : VII Telethon Convention
1999 Rimini (Italy) : VIII Telethon Convention
2000 Rimini (Italy) : IX Telethon Convention
2001 Riva del Garda (Italy) : X Telethon Convention
2002 Riva del Garda (Italy) : XI Telethon Convention
2002 Napoli (Italy) : Third International Workshop “HMGA proteins in cell differentiation and transformation”
2003 Positano (Italy) : V International Meeting of Molecular Oncology
2005 Salsomaggiore (Italy) : XIII Telethon Convention
2005 Positano (Italy) : VI International Meeting of Molecular Oncology
Affiliation :
•
Member of American Diabetes Association
• Member of Italian Endocrine Society
Awards and Personal Grants:
•
1990 Juvenil Diabetes Foundation Research
Grant.
• 1990 Scholarship SIE-SANDOZ,
23° Coference of the Italian Endocrine Society.
• 1992 Endocrinology Under 35
award.
• 1992 Scholarship SIE-SANDOZ
24° Coference of the Italian Endocrine Society.
• 1993 Travel
Grant V° International Symposium on Insulin Receptor and Insulin Action,
Munich (Germany).
• 1997 Telethon-Italy
Grant on “Molecular genetics of insulin resistant diabetes mellitus”.
• 2002 Cofin-MURST Grant on “Molecular
role of HMGA1 in human insulin receptor expression and function”.
• 2004 Cofin-MURST
Grant on “Molecular role of HMGA1 in the pathophysiology of insulin
action”.
• 2004 Telethon-Italy
Grant on “Molecular genetics of insulin resistant diabetes mellitus”.
Selected Publications:
1. P. Marchetti, L. Benzi, V. Trischitta, A. Brunetti, P. Cecchetti, A.M. Ciccarone, V. Pezzino, R. Vigneri, R. Navalesi: “Complementary use of gel permeation and reverse-phase liquid chromatography for the analysis of A14-125I-insulin and its degradation products in isolated human monocytes”. J. of Chromatography, Biomedical Applications 377: 339-344, 1986.
2. V. Trischitta, L. Benzi, A. Brunetti, P. Cecchetti, R. Vigneri, R. Navalesi: “Intracellular insulin processing is altered in monocytes from patients with type 2 diabetes mellitus” J. Clin. Endocrinol. Metab. 64: 914-920, 1987
3. F. Purrello, D. Gullo, A. Brunetti, M. Buscema, S. Italia, I.D. Goldfine, and R. Vigneri: “Direct effects of biguanides on glucose utilization in vitro” Metabolism 36: 774-776, 1987
4. D.M. Hawley, B.A. Maddux, K.Y. Wong, A. Brunetti, V. Trischitta, I.D. Goldfine: “Mechanisms for insulin receptor transmembrane signalling”. In Excerpta Medica, Elsevier Publisher B.V. (Biomedical Division). Diabetes 1988. R.G. Larkins, P.Z. Zimmet, D.J. Chisholm, editors: 213-216, 1988.
5. A. Brunetti, B.A. Maddux, K.Y. Wong, and I.D. Goldfine: “Muscle cell differentiation is associated with increased insulin receptor biosynthesis and messenger RNA levels” J. Clin. Invest. 83: 192-198, 1989
6. D.M. Hawley, B.A. Maddux, R.G. Patel, K.Y. Wong, P.W. Mamula, G.L. Firestone, A. Brunetti, E. Verspohl, and I.D. Goldfine: “Insulin receptor monoclonal antibodies that mimic insulin action without activating tyrosine kinase” J. Biol. Chem. 264: 2438-2444, 1989
7. V. Trischitta, K.Y. Wong, A. Brunetti, R. Scalisi, R. Vigneri, and I.D. Goldfine: “Endocytosis, recycling, and degradation of the insulin receptor” J. Biol. Chem. 264: 5041-5046, 1989
8. V. Pezzino, V. Papa, V. Trischitta, A. Brunetti, .A. Goodman, M.K. Treutelaar, J.A. Williams, B.A. Maddux, R. Vigneri, and I.D. Goldfine: “Human insulin receptor radioimmunoassay: applicability to insulin-resistant states” Am. J. Phisiol. 257: E451-E457, 1989
9. V. Trischitta, A. Brunetti, A. Chiavetta, L. Benzi, V. Papa, and R. Vigneri: “Defects in insulin-receptor internalization and processing in monocytes of obese subjects and obese NIDDM patients” Diabetes 38: 1579-1584, 1989
10. A. Brunetti, B.A. Maddux, K.Y. Wong, C. Hofmann, J. Whittaker, C. Sung, and I.D. Goldfine: “Monoclonal antibodies to the human insulin receptor mimic a spectrum of biological effects in transfected 3T3/HIR fibroblasts without activating receptor kinase” Biochem. Biophys. Res. Commun. 165: 212-218, 1989
11. P.W. Mamula, A.R. McDonald, A.Brunetti, Y. Okabayashi, K.Y. Wong, B.A. Maddux, C. Logsdon, and I.D. Goldfine: “Insulin receptor gene expression is regulated by differentiation and hormones”. In Frontiers in Diabetes Research. Lessons from animal diabetes III. Ed. E. Shafrir, Vol. 1: 242-248, 1990.
12. P.W. Mamula, A.R. McDonald, A. Brunetti, Y. Okabayashi, K.Y. Wong, B.A. Maddux, C. Logsdon, and I.D. Goldfine: “Regulation of insulin-receptor gene expression by differentiation and hormones” Diabetes Care 13: 288-301, 1990
13. P. Sbraccia, K.Y. Wong, A. Brunetti, R. Rafaeloff, V. Trischitta, D.M. Hawley, and I.D. Goldfine: “Insulin downregulates insulin receptor number and upregulates insulin receptor affinity in cells expressing a tyrosine defective insulin receptor” J. Biol. Chem. 265: 4902-4907, 1990
14. A. Brunetti, I.D. Goldfine: “Role of myogenin in myoblast differentiation and its regulation by fibroblast growth factor” J. Biol. Chem. 265: 5960-5963, 1990
15. A. Brunetti, I.D. Goldfine: “Differential effects of fibroblast growth factor on insulin receptor and muscle specific protein gene expression in BC3H-1 myocytes” Mol. Endocrinol. 4: 880-885, 1990
16. V. Papa, C.C. Reese, A. Brunetti, R. Vigneri, P.K. Siiteri, and I.D. Goldfine: “Progestins increase insulin receptor content and insulin stimulation of growth in human breast carcinoma cells” Cancer Research 50: 7858-7862, 1990
17. L. Benzi, V. Trischitta, A.M. Ciccarone, P. Cecchetti, A. Brunetti, S. Squatrito, P. Marchetti, R. Vigneri, and R. Navalesi: “Improvement with metformin in insulin internalization and processing in monocytes from NIDDM patients” Diabetes 39: 844-849, 1990
18. S.M. Rosenthal, A. Brunetti, E.J. Brown, P.W. Mamula, and I.D. Goldfine: “Regulation of insuli-like growth factor (IGF)I receptor gene expression during muscle cell differentiation: potential autocrine role of IGF-II” J. Clin. Invest. 87: 1212-1219, 1991
19. S.M. Rosenthal, E.J. Brown, A. Brunetti, and I.D. Goldfine: “Fibroblast growth factor inhibits insulin-like growth factor-II (IGF-II) gene expression and increases IGF-I receptor abundance in BC3H-1 muscle cells” Mol. Endocrinol. 5: 678-684, 1991
20. R. Rafaeloff, B.A. Maddux, A. Brunetti, P. Sbraccia, C.K. Sung, R. Patel, D.M. Hawley, I.D. Goldfine: “Transmembrane signalling by insulin via an insulin receptor mutated at tyrosines 1158, 1162 and 1163”. Biochem. Biophys. Res. Commun. 179: 912-918, 1991
21. P.A. Goodman, P. Sbraccia, A. Brunetti, K.Y. Wong, J.D. Carter, S.M. Rosenthal, I.D. Goldfine: “Growth factor receptor regulation in the minn-1 leprechaun: defects in both insulin receptor and EGF receptor gene expression” Metabolism 41(5): 504-509, 1992
22. C.K. Sung, X-L. Han, A. Brunetti, B.A. Maddux, R. Yamamoto-Honda, and I.D. Goldfine: “Regulation of biological functions by an insulin receptor monoclonal antibody in insulin receptor beta-subunit mutants” Biochemistry 31: 168-174, 1992
23. A. Brunetti, D. Foti, and I.D. Goldfine: “Identification of unique nuclear regulatory proteins for the insulin receptor gene which appear during myocyte and adipocyte differentiation” J. Clin. Invest. 92: 1288-1295, 1993
24. A. Brunetti, D. Foti, K.Y. Wong, R. Vigneri, and I.D. Goldfine: “Insulin receptor gene expression is regulated by differentiation”. In New Perpectives in Endocrinology. Ares-Serono Symposia Series, Raven Press 99: 395-400, 1993.
25. A. Brunetti, Ira D. Goldfine: “Insulin receptor gene expression and insulin resistance”. Review Article J. Endocrinol. Invest. 18: 398-405, 1995
26. A. Brunetti, L. Brunetti, D. Foti, D. Accili, I.D. Goldfine: “Human diabetes associated with defects in nuclear regulatory proteins for the insulin receptor gene” J.Clin. Invest. 97: 258-262, 1996
27. A. Brunetti, E. Chiefari, S. Filetti, and D. Russo: “The 3’,5’-cyclic adenosin monophosphate response element binding protein (CREB) is functionally reduced in human toxic thyroid adenomas” Endocrinology 141: 722-730, 2000
28. A. Brunetti, G. Manfioletti, E. Chiefari, I.D. Goldfine, and D. Foti: “Transcriptional regulation of human insulin receptor gene by the high-mobility group protein HMGI(Y)” FASEB J 15: 492-500, 2001
29. E. Chiefari, A. Brunetti, F. Arturi, JM Bidart, D. Russo, M. Schlumberger, S. Filetti. “Increased expression of AP2 and Sp1 transcription factors in human thyroid tumors: a role in NIS expression regulation?”. BMC Cancer. 2:35, 2002.
30. D. Foti, R. Iuliano, E. Chiefari, and A. Brunetti. “A nucleoprotein complex containing Sp1, C/EBP-beta, and HMGI-Y controls human insulin receptor gene transcription” Mol Cell Biol. 23: 2720-2732, 2003
31. M.L. Hribal, L. Perego, S. Lovari, F. Andreozzi, R. Menghini, C. Perego, G. Finzi, L. Usellini, C. Placidi, C. Capella, V. Guzzi, D. Lauro, F. Bertuzzi, A. Davalli, G. Pozza, A. Pontiroli, M. Federici, R. Lauro, A. Brunetti, F. Folli, G. Sesti. “Chronic hyperglycemia impairs insulin secretion by affecting insulin receptor expression, splicing, and signaling in RIN beta cell line and human islets of Langerhans FASEB J 17:1340-1348, 2003
32. C. D'Alessandris, F. Andreozzi, M. Federici, M. Cardellini, A. Brunetti, M. Ranalli, S. Del Guerra, D. Lauro, S. Del Prato, P. Marchetti, R. Lauro, G. Sesti. “Increased O-glycosylation of insulin signaling proteins results in their impaired activation and enhanced susceptibility to apoptosis in pancreatic beta-cells. FASEB J. 18: 959-61, 2004
33. D. Foti, E. Chiefari, M. Fedele, R. Iuliano, L. Brunetti, F. Paonessa, G. Manfioletti, F. Barbetti, A. Brunetti, C.M. Croce, A. Fusco, A. Brunetti. “Lack of the architectural factor HMGA1 causes insulin resistance and diabetes in humans and mice. Nat. Med 11: 765-773, 2005
