Dr. Guebel Daniel Víctor

Name: Guebel, Daniel Víctor
Born: 25/09/54

Address:
Av. San Martín 4927 dpto A.
City: Buenos Aires
Postal Code: 1417
Country: Argentina
Tel: +(54)11 4503-9355

::. ACADEMIC DEGREES

Doctorate in Biochemistry (Biotechnology):
Buenos Aires University. Argentina (1992).

Calification: 10 points.
Subject: "Contribución al estudio de la producción de etanol a partir de residuos hemicelulósicos mediante un modelo experimental Pichia stipitis-D-xilosa".

Thesis Director: Dr. Clara Nudel.

This thesis was laureate with the Accesit Award of the Biochemistry School. Buenos Aires National University. Argentina (1994).

Master in Pharmaceutical-Biochemistry Industries:
School of Biochemistry. Buenos Aires University. Argentina (1988).

Biochemist.
School of Biochemistry. Buenos Aires University. Argentina (1978)

::. Present Position:

Researcher member of the Biochemistry Technologies Group. Department of Biochemistry and Molecular Biology. La Laguna University. Tenerife. Spain. Director of the Biotechnology Counseling Services. Buenos Aires. Argentina.

I have acted as reviewer for several scientific journals, such as Biotechnology Bioengineering (2000), Microbial Research (2004), and Electronic Journal Biotechnology (2005).

::. ACADEMIC ANTECEDENTS

Teaching at the University (Grade Courses):

• Associated Professor of Metabolic Engineering. Science and Technology Department. Biotechnology School. Quilmes National University. Argentina (2000).

• Associated Professor of BioProcess I and II. Science and Technology Department. Biotechnology School. Quilmes National University (1998-2000).

• Associated Professor of Experimental Design. Science and Technology Department. Biotechnology School. Quilmes National University (1998).

• Lecturer Professor (1996-97) and Associated Professor (1997-98) of Special Microbiology. Science and Technology Department. Biotechnology School. Quilmes National University.

• Lecturer Professor (1996-97) and Associated Professor (1997-98) courses of Design of Biotechnology Processes. Science and Technology Department. Biotechnology School. Quilmes National University.

• Chief for laboratory activities at the Biotechnology and Industrial Microbiology Laboratory. School of Biochemistry. Buenos Aires University. (1990-1994).

• Graduated Assistant of Biotechnology and Industrial Microbiology courses. Biochemistry School. Buenos Aires University. (1989-1990).

Teaching at the University (Post Graduate Courses)

• Director and Professor of doctorate course “An Introduction to Metabolic Engineering and their applications to Biotechnology”. Science and Technology Department. Biotechnology School. Quilmes National University (1999) [The course was dictated in collaboration with Dr. Néstor Torres, Spain]

• Director and Professor of course “An Introduction to Metabolic Engineering”. Science and Technology Department. Biotechnology School. Quilmes National University (1998). [The course was dictated in collaboration with Dr. Néstor Torres, Spain]

• Director and Professor of course “Modeling and Simulation as a Tool of Research and Development in Biotechnology”. Science and Technology Department. Biotechnology School. Quilmes National University. (1995).

• Professor at the First PostGraduate International Course about Microorganisms Interactions with its ambient. School of Biochemistry. Buenos Aires University and La Plata University under the direction of Dres. Hellinwerf K. y Neijssel O. (Amsterdam University) (1993).

Professional Post Graduated Courses Given

STATISTICS APPLIED TO QUALITY CONTROL. Argentina Biochemistry Association (1984, 1985); OPERATIVE SECURITY COURSES (1983, 1984).

::. R.R.H.H FORMATION

• Director of research fellowship and doctoral thesis of Lic. Sebastian Oddone. National Quilmes University.
Subject: “OPTIMIZATION ESTRATEGIES FOR THE EXPRESSION OF RECOMBINANT PROTEINS” (Period 1999-2000).

• Director of research fellowship of Lic. Sebastian Oddone. National Quilmes University.
Subject: “OPTIMIZATION OF METALOPROTEINASE HUMAN INHIBITOR RECOMBINANT PRODUCTION FROM INSECT CULTURE CELLS” (March 1998-December 1999).

• Director of degree thesis of Sebastián Oddone. National Quilmes University. (Period 1997-1998).
Subject: “EXPERIMENTAL DIAGNOSTIC OF SOME PROBLEMS INVOLVED IN INOCCULANT PRODUCTION FOR LEGUMINOSAS”. Approved with maximal calification (10 points). March 1998.

• Director of research fellowship of the student Sebastian Oddone. National Quilmes University.
Subject: “OPTIMIZATION OF RECOMBINANT PROTEIN EXPRESSION FROM SPODOPTERA FRUGIPERDA CULTURE CELLS” (Period 1997-1998).

• Director of fellowship of student Gustavo F. Mahler. Biochemistry School. National Buenos Aires University.
Subject: "ESTRATEGIES FOR INCREASING ETHANOL PRODUCTIVITY BY THE MANIPULATION OF MAINTENACE CELLULAR ENERGY" (1992-1993).

• Director of fellowship students Posse Graciela and Cieza Lucio at the Bio-Process Laboratory. Science and Technology Department. National Quilmes University (1999-2000).

• Director of Graduate Laboratory Assistants of Bio-Process Courses, Lic. Mariana Nores (1998-1999); Lic. Sebastian Oddone. UNQ. (Period 1998-2000); Lic. Víctor Inchaurrondo (1996-1998); Director of Graduate Assistants of Experimental Design Course, Lic. Ernesto Aljinovic (1998).

• Director of laboratory assistants of BioProcess Laboratory. National Quilmes University. Lic. Gustavo Janica. (Period 1998-2000) and the students Diego Faccone (1998-1999); Médici Rosario, Ferela Marcela, Portal Maximiliano, Agostino Patricia (2000).

::. POST DOCTORAL VISITS

» Training in Metabolic Engineering techniques with Dr. Néstor V. Torres Darias. Laboratorio de Control Metabólico y Tecnologías Bioquímicas. Dpto. Biología Molecular. Facultad de Biología. Universidad de La Laguna. Tenerife. Spain. (January and February 1998).

» Training in Metabolic Engineering techniques with Dr. Iosse Texeira de Mattos. Microbiology Department. Amsterdam University. The Netherlands. (1–10 march 1998).

» Training and research working with Dr. José La Torre at the Animal Virology Centre (CEVAN-CONICET) Argentine. Subject: “Optimization of recombinant protein production from insect cell cultures”. May-December 1995.

::. Other Relevant Activities

• Consultant and development of productive process for Fragaria SRL (Argentina), producer of microbial inoculants for agriculture (2004).

• Consultant of the Organización Estados Iberoamericanos (OEI) (2002)

• Consultant of the Comisión Nacional de Evaluación y Acreditación Universitaria. Ministerio de Educación. Argentina (2001).

• Member of the Scientific Committee in the VI International Symposium on Biochemical Systems Theory. Puerto de la Cruz. Tenerife. Spain. (Sept. 25-29, year 2000).

• Coordinator of the Biotechnology Area. Department of Science and Technology. Quilmes. National Univerity. Argentina (1997-2000).

• Member of the Comission for the reformulation of the curricula for the Biotechnology degree. National Quilmes Univeristy. Argentina (1996-1998).

• Development of interactive activities between BioProcess Laboratory and the Automation Engineering Group of National Quilmes University (1999).

• Development of computerized tutorial system to teach Experimental Design. National Quilmes University (1998).

• Consultant about productive process to obtain microbial inoculants for agriculture. Contract between Síntesis Química and National Quilmes University (1997-1998).

• Consultant and development of productive process to obtain bioinsecticides for PRAREX TRADE S.A (1994).

• Consultant about productive process for PROGALEN S.A producer of medical esteril materials (1993).

• Consultant about productive process for BIOIM SRL producer of medical esteril materials (1993).

• Development of productive process and implementation of microbiological control laboratory for ESPRO S.A. (Bs.As / Miramar). Zorraquín Group (1991).

• Research working. Contract between Biotechnology Laboratory of Biochemistry School of Buenos Aires University and the Paul Laboratory of Argentina (1987-1989).

• Laboratory Chief. UTA Medical Center. Argentina (1980-1995).

::. MANAGEMENT OF RESEARCH PROYECTS

» Channeling of GAD-65 human protein expression in Escherichia coli toward a soluble form appropriated for its massive application in humane medicine. Approved without funds. FONCYT. PICYT. Argentina. (1999).

» Optimization of the obtention of GAD-65 human recombinant protein soluble in Escherichia coli. National Quilmes University. Programme MESIB (1999-2000).

» Obtention of Metaloproteinase human inhibitor from insect cells culture. National Quilmes University (1998-1999).

» Optimization of obtention of recombinant proteins from insect cell culture. National Quilmes University. Co Director Project (1996-1998).

::. FELLOWSHIP

• Grant of 15.000$. FOMEC-535. Education Agency. Argentina (1997).

• INCE Fellowship during university studies. Education Office Ministery. Argentina

INVITED CONFERENCES

• Appplication of multivariate and chemometrics tools to solve environmental problems. Campomar Foundation and Faculty of Sciences. Buenos Aires (December 2004)

• Analysis of the specialties oils production improvement in oleaginous from a metabolic engineering point view. Zeneca International. Balcarce. Argentina (March 1999).

• A picture of possible biotechnological applications of metabolic engineering.
Genetic Institute- National Institute of Agriculture Technologies (INTA Castelar). Argentina. (December, 1998).

AWARDS

Accesit Biochemistry School. National Buenos Aires University. (1994)

SCIENTIFIC CONGRESS PARTICIPATION

» “Optimization of the citric acid production by A. niger through a mass and energy balance model”. Daniel V. Guebel (Universidad Nacional de Quilmes, Argentina) y Néstor V. Torres (ULL, España). VI International Symposium on Biochemical Systems Theory. Puerto de la Cruz. Tenerife. Spain. Sep 25/29, 2000.

»“Desarrollo de un modelo integrativo para la idiophase del hongo Aspergillus niger”. Daniel V. Guebel (UNQ, Argentina) y Néstor V. Torres (ULL, España). XXXV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. (SAIB). Nov. 1999.

»“Localización de posibles targets suceptibles de ser modificados por ingeniería metabólica en Aspergillus niger para la optimización de la producción de ácido cítrico”. Daniel V. Guebel (UNQ, Argentina) y Néstor V. Torres (ULL, España). XXXV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular. (SAIB). Nov. 1999.

» “Estudio de factibilidad económica del proceso de producción de la proteína GAD recombinante para su aplicación masiva en medicina humana”. Pose G, Ciezca L.A., Medici R., Guebel D.V. Primeras Jornadas de Tesistas de Posgrado. UNQ.1999.

» “Desarrollo de cultivos de alta densidad para la producción optimizada de proteína GAD recombinante humana en Escherichia coli”. Cieza L.A., Pose G., Medici R., Guebel D.V. Primeras Jornadas de Tesistas de Posgrado. UNQ. 1999.

» “Scale down de la condiciones para obtener cultivos de alta densidad de Escherichia coli recombinante GI698 pTrx-GAD mediante estrategias sistemáticas de experimentación estadística”. Pose G., Cieza L.A. , Medici R., Guebel D.V. Primeras Jornadas de Tesistas de Posgrado. UNQ. 1999.

» “Stoichiometric analysis of Spodóptera frugiperda (SF-9) cells in agitated culture”. Guebel D.V. (UNQ.Argentina) and Torres N.V. (U.L.L. España). International Symposium in Power-Law Modelling of Biological Systems. Oerias. Portugal. 1998.

» “Efecto del Magnesio en el crecimiento y producción de etanol por Pichia stipitis”. Mahler G.F., Guebel D.V., Giulietti A.M. 3erCongreso Latino Americano de Biotecnología. Chile. 1993.

» Efecto del Magnesio y las vitaminas en el crecimiento y la producción de etanol por Pichia stipitis. Mahler G.F, Giulietti A.M., Guebel D.V. XXVIII Reunión Anual de SAIB. Huerta Grande (Pcia.Córdoba). 1992.

» Is it possible to increase ethanol productivity in P.stipitis by energetic stress?. Guebel D.V., Nudel B.C., Giulietti A.M. 9th International Biotechnology Symposium. Washington. 1992.

» “Estudio sobre las condiciones apropiadas de floculación en la levadura Pichia stipitis NRRL Y 7124”. Guebel D.V., Nudel B.C., Giulietti A.M . XI Congreso Latino Americano de Microbiología.Bs.As. 1991.

» “Influencia de la fuente de nitrógeno sobre la capacidad de crecimiento y fermentaiva de de Pichia stiptis NRRL Y 7124”. Guebel D.V.,Cordenons A.A,Cascone O.,Nudel B.C.,Giulietti A.M. XI Congreso Latino Americano de Microbiología. Bs.As. 1991.

» Actividad Macrofágica y Anestésicos Halogenados. Jorrnadas Científicas Hospital Pirovano. 1988.Guebel D.V, Herzage León.

» Fermentation of D Xylose by Pichia stipitis. 7th International Symposium on Yeast.Perugia. Italia. 1988. Guebel D.V., Giulietti A.M

PUBLICATIONS

» Modeling early events in colon tumorogenesis. Guebel D.V (Argentina), Torres N.V. (ULL, Spain), (in preparation)

» Modeling analysis of the process for L-(-)-carnitine production by Escherichia coli. Guebel D.V (Argentina), Torres N.V. (ULL, Spain), Cánovas M (U.M., Spain). (accepted in Process Biochemistry, Aug., 2005).

» Interactive Astrocytic-Neuronal Sulphur Amino Acid Metabolism in mammanlian brain: its assesment by a Mathematical Hybrid Model. Guebel D.V. (Argentina) & Torres N.V. (ULL, España) (2004). Biochimica et Biophysica Acta (general subjects), 1674:12-28

» Canonical sensitivities: A useful tool for dealing with large system perturbations in metabolic modeling. Guebel D.V. (2004). In Silico Biology, 4, 0015. Available in: http://www. bioinfo.de/isb/2004/04/0015

» Optimization of the citric acid production by A. niger through a flux balancing model. Guebel D.V. & Torres N.V. (ULL, España) (2001) EJB: Electronic Journal of Biotechnology [on line]. Availanble in: http://www.ejbiotechnology.info/content/vol4/issue1/full2. ISSN: 0717-3458.

» Influence of Magnesium ion on growth, ethanol and xylitol production by Pichia stipitis NRRL 7124. Mahler G.F. and Guebel D.V. (1994). Biotechnology Letters, 16:407-412.

» Determination of the Settling Velocity Distribution of Cell Aggregates by a Standardized Spectrophotometric Method. Guebel D.V. & Nudel B.C. (1994). Biotechnology Techniques, 8(8):529-534.

» Antagonism between growth and flocculation in Pichia stipitis NRRL-7124: Influence of Ca+2 and Mg+2 ions. Guebel D.V. & Nudel C.B. (1994). Biotechnology Letters,16:143- 148.

» Influence of the Nitrogen Source on Growth and Ethanol Production by Pichia stipitis NRRL Y 7124. Guebel D.V., Cordenons A, Cascone O.,Giulietti A.M. & Nudel C. (1992). Biotechnology Letters.,14(12): 1193-1198.

» A Simple and Rapid Micro Kjeldahl Method for Total Nitrogen Analysis. Guebel D.V., Nudel B.C., Giulietti A.M. (1991) Biotechnology Techniques, 6: 427 43O.

» Influence of Oxygen Transfer Rate and Media Composition on Fermentation of D Xylose by Pichia stipitis NRRL Y 7124. Guebel D.V., Cordenons A.A., Nudel B.C., Giulietti A.M. (1991) Journal Industrial Microbiology, 7: 287 292.

» Xylose Fermentation by Pichia stipitis. Guebel D.V., Cordenons A.A., Nudel B.C, Giulietti A.M. (1989).Yeast (S.I): 73 77

OTHER RELEVANT ANTECEDENTS

• Reviewer of the Electronic Journal in Biotechnology (2005)

• Reviewer of the journal Research in Microbiology (ex Annals of Institute Pasteur) (2004)

• Reviewer of the journal Biotechnology and Bioengineering (2000)

• Member of Scientific Advisory Comitee at the VI International Symposium on Biochemical Systems Theory (SymBioSys). Puerto de la Cruz. Tenerife. Spain (2000).

• Coordinator of the Biotechnology Area. Science and Technology Department. National Quilmes National University. Argentina (1997-2000).

• Member of Department Comission for the Curricular Change. National Quilmes University. Argentina. (1996-1998).

• Laboratory Chief at the Medical Center UTA, Buenos Aires (1979-1995).

• Technical Subsecretary of Biochemistry School. National Buenos Aires University (1992-1993).

• Member Operative Seccurity Comission. Argentine Biochemistry Association (1982-1985).

POST GRADUATE COURSES TAKEN

» PROCESS CONTROL SYSTEMS BY VISUAL BASIC PC-INTERFACES. Engineering School. Technological National University. (2000). Duration: 54 hours.

» BIOLOGICAL SYSTEMS MODELING AND SIMULATION. Caece University. (1989). End Evaluation. Duration: 13O hours.

» BIOTECHNOLOGICAL INTEGRATED PROCESS (1988). Escuela Argentina Brasileña de Biotecnología. Curitiba. Brasil. End Evaluation. Duration: 40 hours.

» International Post Graduate Course on "FERMENTATION TECHNOLOGY". Biochemistry School. Buenos Aires National University. (1987). End Evaluation. Duration: 40 hours.

» Regular courses during master in Biotechnology Industries (with evaluation): BIOTECHNOLOGY I, BIOTECHNOLOGY II, INDUSTRIAL ORGANIZATION, TECHNICAL DRAWING, SECURITY AND HYGIENE, and INDUSTRIAL MICROBIOLOGY (Period 1986-1988).

» H.P.L.C METHODOLOGY. Biochemistry School. Buenos Aires National University. (1988). End Evaluation. Duration: 60 hours.

» INTRODUCTION TO RHEOLOGY APLICATIONS. Biochemistry School. Buenos Aires National University. (1988). End Evaluation. Duration: 60 hours.

» NON LINEAL REGRESSIONS. Biochemistry School. Buenos Aires National University (1987). End Evaluation. Duration: 60 hours.

» INSTRUMENTATION IN CHEMISTRY. School of Chemistry. Buenos Aires National University. (1985). End Evaluation. Duration: 120 Hours.

» BASIC PROGRAMATION. Engineering School. Technological National University. (1985). End Evaluation. Duration: 60 hours.

» RADIOISOTOPES METHODOLOGY. School of Biochemistry. End Evaluation. Duration: 4OO hours. (1982).

» STATISTICS METHODS IN RESEARCH. Biochemistry School. Buenos Aires National University. (1982). End Evaluation. Duration: 120 Hours.

» Training Concurrence Bacteriology Laboratory. Hospital "JOSE DE SAN MARTIN", Buenos Aires National University. Period 1977-78.

OTHER POST GRADUATE COURSES TAKEN

» INTRODUCTION TO RESEARCH METHODOLOGY. Italian Hospital. (1984).

» INSTRUMENTATION. Argentinen Biochemistry Association. (1982).

» SYMPOSIUM ON HIPER LIPOPROTEINS. (1981).

» BIOCHEMISTRY IN GINECOLOGY (1981).

» LATINO AMERICAN SYMPOSIUM ON CLINICAL DIAGNOSTIC BY PROTEINS (1981).

» ENZIMOLOGY IN CLINICS. Foundation Santa Juana de Arco. (1980).

» ELECTROPHORESIS AND INMUNOTECHNIQUES IN THE PHYSIOPATHOLOGY OF PROTEIN DISEQUILIBRIUM. Chemetrón. (1980)

» INFECTIOUS DISSEASES. Medicine School. Buenos Aires National University. (1979).

» GONOCCOCIAL PATHOLOGY. National Institute of Microbiology Dr.Carlos Malbrán. (1979).

» ANGINAS:"PRINCIPALES AGENTES ETIOLOGICOS BACTERIANOS". National Institute of Microbiology Dr.Carlos Malbrán. (1979).

» ANTIBIOTICS TERAPEUTICS. Medicine School. Buenos Aires National University. (1978).

» CLINICAL BACTERIOLOGY. Biological Analysist Association. (1977).

» BIOLOGICAL BASIS OF TERAPEUTICS IN INFECTOUS DISSEASES. American Pediatry Academy. (1977).

» INMUNOLOGICAL BASIS OF TERAPEUTICS IN INFECTOUS DISSEASES. American Pediatry Academy (1977).